- HCM089 R2A Agar
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CATEGORY :
Dehydrated Culture Media
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USAGE :
For enumeration of heterotrophic bacteria in water.
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SPECIFICATION :
500g/bottle
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CODE :
HCM089
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HCM089 R2A Agar
Usages:
For enumeration of heterotrophic bacteria in water.
Principle:
Yeast extract, peptone, provide nitrogen acid protein hydrolysates, vitamins, growth factors; glucose, to fermentable sugars; soluble starch can absorb the harmful substances produced by bacteria in the recovery process; sodium pyruvate may enhance recovery of bacteria. PH buffer is potassium dihydrogen phosphate; provided over anhydrous magnesium divalent cations; agar as coagulant.
Formulation(per liter):
Yeast extract powder 0.5g
Peptone 0.5g
Casein hydrolyzate 0.5g
Glucose 0.5g
Soluble starch 0.5g
Potassium dihydrogen phosphate 0.3g
Anhydrous magnesium sulfate 0.024g
Sodium pyruvate 0.3g
Agar 15.0g
Final pH 7.2 ± 0.2
How to use:
1.Suspend 18.1g in 1 L of distilled water , stirring heated to boiling until completely dissolved, dispensing flask, 121 ℃ autoclave for 15min.
2.Diluted and treated samples.
Quality control:
The name and number of strain |
Indicator |
Culture Time & Temperature |
Colony |
Bacillus subtilis CMCC(B)63501 |
Promote growth ability |
24h,30-35℃
Good |
PR≥0.7,white colony
|
Pseudomonas aeruginosa CMCC(B)10104 |
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Specifications: 500g/bottle
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