- 027043P1 Cetrimide Agar Base(Granular Culture Media)
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CATEGORY :
Granular Culture Media
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USAGE :
For isolation and enumeration of Pseudomonas aeruginosa in water samples.
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SPECIFICATION :
250g/bottle
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CODE :
027043P1
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027043P1 Cetrimide Agar Base(Granular Culture Media)
Usages:
For isolation and enumeration of Pseudomonas aeruginosa in water samples.
Principle:
Peptone provide nitrogen; glycerol carbon source; potassium and magnesium can promote green pus pigment production; hexadecyl trimethyl ammonium bromide is a selective antibacterial agent, as a quaternary ammonium salt cationic detergent may bacterial cells release nitrogen and phosphorus and suppress non-Pseudomonas aeruginosa bacteria; agar is medium coagulant.
Formulation(per liter):
Pancreatic Digest of Peptone 20.0g
Magnesium Chloride 1.4g
Dipotassium Sulphate 10.0g
Cetrimide 0.3g
Agar 13.6g
Final pH 7.2 ± 0.2
How to use:
1.Suspend 45.3g in 1L of distilled water , stirring heated to boiling to completely dissolve ,autoclave at 121℃ for 15 minutes.
2.Diluted and treated samples.
Quality control:
Item |
The name and number of strain |
Growth |
Colony Color |
1 |
Pseudomonas aeruginosa ATCC27853 |
Good |
Yellow -green |
2 |
Escherichia coli ATCC25922 |
Inhibition |
-- |
3 |
Staphylococcus aureus ATCC25923 |
Inhibition |
-- |
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Specifications: 250g/bottle
*Supplement:029080 Glycerol
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