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HCM032 Kligler Iron Agar


Dehydrated Culture Media


For differentiation of enterobacteriaceae.





  • MSDS
HCM032 Kligler Iron Agar
For differentiation of enterobacteriaceae.
Peptone, beef extract powder and yeast extract powder provide nitrogen, vitamins, minerals; lactose, dextrose into fermentable sugars, which produce acid when measured by the phenol red indicator, acid yellow, basic red; thiosulfate sodium can be reduced to some bacteria to hydrogen sulfide, to produce a black iron sulfide and iron salts; sodium chloride to maintain osmotic equilibrium; agar as medium coagulant.
Formulation(per liter):
Peptone 20g
Beef extract powder 3g
Yeast extract powder 3g
Lactose 10g
Dextrose 1g
Sodium chloride 5g
Ferric ammonium citrate 0.5g
Sodium thiosulfate 0.5g
Agar 12g
Phenol red 0.05g
Final pH 7.4 ± 0.2
How to use:
1.Suspend 54.5g in 1L of distilled water , stirring heated to boiling ,autoclave at 121℃ for 15 minutes.
2.Diluted and treated samples.
Quality control:
Item The name and number of strain Growth Colony Color
1 Escherichia coli ATCC25922 Good A/A
2 Proteus CMCC (B) 49027 Good K/A 
3 Salmonella typhimurium CMCC (B) 50115 Good K/A
“A”Acid , “K” Alkality
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Specifications: 500g/bottle