- HCM023 Fraser Broth Base
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CATEGORY :
Dehydrated Culture Media
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USAGE :
For selective enrichment of Listeria spp.
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SPECIFICATION :
500g/bottle
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CODE :
HCM023
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HCM023 Fraser Broth Base
Usages:
For selective enrichment of Listeria spp.
Principle:
Casein digest, animal tissue enzyme digests, beef extract powder, yeast extract powder provide nitrogen, vitamins, amino acids and growth factors; sodium chloride maintains osmotic equilibrium; disodium hydrogen phosphate and potassium dihydrogen phosphate buffer agent; Esculin is fermentable sugars; lithium chloride and other antibiotics can inhibit the Gram-negative bacteria and most gram-positive bacteria.
Formulation（per liter）:
Casein digest 5.0g
Animal tissue enzyme digests 5.0g
Beef extract powder 5.0g
Yeast extract powder 5.0g
NaCl 20.0g
Disodium hydrogen phosphate 12.0g
Potassium dihydrogen phosphate 1.35g
Esculin 1.0 g
Lithium chloride 3.0g
Final pH 7.2 ± 0.2
How to use：
1.Suspend 28.7g in 1L of distilled water , stirring heated to boiling ,autoclave at 121℃ for 15 minutes.
2.Diluted and treated samples.
Quality control：
|
Item |
The name and number of strain |
Growth |
Colony Color |
|
1 |
Listeria monocytogenes bacteria CMCC (B) 54002 |
Good |
Black and cloudy |
|
2 |
Escherichia coli ATCC25922 |
Inhibited |
-- |
|
3 |
Streptococcus faecalis CMCC (B) 32223 |
Inhibited |
-- |
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Specifications: 500g/bottle
*Supplement: SR0120( acriflavine hydrochloride, nalidixic acid, ferric ammonium citrate)
SR0130 (criflavine hydrochloride, nalidixic acid)
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FOCUS ON MICROBIAL DETECTION & CONTROL
