- 021140 Linden Grain Medium ( LG Medium)
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CATEGORY :
Dehydrated Culture Media
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USAGE :
For detection of molds, yeasts and total bacteria , commonly used in aseptic filling empty PET bottl
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SPECIFICATION :
10kg/bag; 1kg/bag
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CODE :
021140
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021140 Linden Grain Medium ( LG Medium)
Linden Grain Broth
Usages:
For detection of molds, yeasts and total bacteria , commonly used in aseptic filling empty PET bottle production line for sterility testing.
Principle: peptone and yeast extract powder provides carbon and nitrogen sources and trace elements; glucose as carbon source; potassium dihydrogen phosphate as a buffer; ammonium sulfate osmotic equilibrium can be maintained and conducive to the growth of target bacteria.
Formulation (per liter):
Glucose :20.0g
Ammonium sulfate:2.0g
Magnesium sulfate:1.0g
Peptone:2.0g
Yeast extract powder: 3.5g
Sodium dihydrogen phosphate: 1.0g
Final pH 6.5 ± 0.2
How to use:
1. Weight 29.5g of product, adding 1L of distilled water or deionized water,stirring heated to boiling until complete dissolution, dispensing tube or flask, autoclave at 121 ℃ for 15minutes.
2. Inoculated the treated sample to LG medium, according to the specific requirements of sterility testing, vaccination appropriate sample size, amount of medium and positive comparison ,culture at 28 ℃ ~ 32 ℃ for 13 ~ 15d.
3. Observe the results.
Quality Control:
Appearance:
Powder: light yellow powder
Prepared broth: yellow, clear and transparent without precipitation.
Biology testing: The following strains were inoculated and cultured at 28 ℃ ~ 32 ℃ for 13 ~ 15d ,growth as below:
Quality control
Strain Name Strain Code growth characteristics
Pseudomonas aeruginosa ATCC27853 turbid broth
Staphylococcus aureus ATCC6538 turbid broth
Bacillus cereus ATCC63303 turbid broth
Klebsiella pneumonia ATCC46117 turbid broth
Hyphae of Aspergillus niger ATCC16404 flocculent, liquid black spores
Candida albicans ATCC10231 turbid broth
Storage: Store in a dark, cool and dry place, sealed the product immediately after use.
Storage period of three years
Specifications: 021140A 10kg/bag 021140B 1kg/bag
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FOCUS ON MICROBIAL DETECTION & CONTROL
