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HCM109 Simmons Citrate Agar

CATEGORY :

Dehydrated Culture Media

USAGE :

For the differentiation of Gram-negative bacteria on the basis of citrate utilization.

SPECIFICATION :

500g/bottle

CODE :

HCM109

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  • MSDS
HCM109 Simmons Citrate Agar
 
Usages:
For the differentiation of Gram-negative bacteria on the basis of citrate utilization.
 
Principle:
Magnesium ions in various metabolic cofactors; ammonium dihydrogen phosphate to provide nitrogen; dipotassium hydrogen phosphate is the buffer; sodium citrate as a carbon source; agar as medium coagulant.
 
Formulation(per liter):
Sodium chloride 5g
Magnesium sulfate 0.2g
Ammonium dihydrogen phosphate 0.2g
Sodium ammonium phosphate 0.8g
Sodium citrate 2g
Agar 15g
Bromothymol blue 0.08g
Final pH 7.0 ± 0.2
 
 
How to use:
1.Suspend 23.3g in 1 L of distilled water , stirring heated to boiling until completely dissolved, dispensing flask, 121 ℃ autoclave for 15min.
2.Diluted and treated samples.
 
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
 
Specifications: 500g/bottle