- HCM071 Violet Red Bile Agar(VRBA)
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CATEGORY :
Dehydrated Culture Media
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USAGE :
For coliform plate count of water or food sample.
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SPECIFICATION :
500g/bottle
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CODE :
HCM071
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HCM071 Violet Red Bile Agar with Glucose and Lactose
Usages:
For seletive isolation of Enterobacteriaceae.
Principle:
Peptone and yeast extract powder provides carbon and nitrogen sources and trace elements; lactose are fermentable sugars; sodium chloride maintains osmotic equilibrium; the 3rd bile salts and crystal violet inhibiting gram-positive bacteria, especially against Gram positive bacteria and fecal streptococci; neutral red as pH indicator.
Formulation（per liter）:
Yeast extract powder 3g
Pancreatic digest of gelatin 7g
Bile salt 1.5g
Lactose 10g
Sodium chloride 5g
D-Glucose Monohydrate 10g
Neutral Red 0.03g
Crystal violet 0.002g
Agar 15g
Final pH 7.4 ± 0.2
How to use：
1.Suspend 51.5g in 1 L of distilled water , stirring heated to boiling until completely dissolved, Do not autoclave.
2.Diluted and treated samples.
Quality control：
|
Item |
The name and number of strain |
Growth |
Colony Color |
|
1 |
Escherichia coli ATCC25922 |
Good |
Dark red |
|
2 |
Aerogenes CMCC (B) 45103 |
Good |
red |
|
3 |
Staphylococcus aureus ATCC6538 |
Suppressed |
-- |
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Specifications: 500g/bottle
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FOCUS ON MICROBIAL DETECTION & CONTROL
